Second Generation Multiplex

Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR (Short Tandem Repeat) loci: VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), and FGA (HUMFIBRA). It also contains primers for the Amelogenin sex indicating test. The primers are tagged with the following fluorescent dyes for detection under electrophoresis: 5-FAM, JOE, and NED. Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM+ DNA profiling system in 1998.

This article has multiple issues. Please help improve it or discuss these issues on the talk page. (Learn how and when to remove these messages) This article relies largely or entirely on a single source. Relevant discussion may be found on the talk page. Please help improve this article by introducing citations to additional sources. Find sources: "Second Generation Multiplex" – news · newspapers · books · scholar · JSTOR (November 2025) This article may be too technical for most readers to understand. Please help improve it to make it understandable to non-experts, without removing the technical details. (June 2012) (Learn how and when to remove this message) (Learn how and when to remove this message)

Second Generation Multiplex is a DNA profiling system used in the United Kingdom to set up the UK National DNA Database in 1995.^[1] It is manufactured by ABI (Applied Biosystems).

It contains primers for the following STR (Short Tandem Repeat) loci: VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), and FGA (HUMFIBRA).

It also contains primers for the Amelogenin sex indicating test.

The primers are tagged with the following fluorescent dyes for detection under electrophoresis: 5-FAM, JOE, and NED.

Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM+ DNA profiling system in 1998.

References

[edit]

1. ^ Gill, Peter (2014-01-01), Gill, Peter (ed.), "Chapter 4 - National DNA Databases, Strength of Evidence and Error Rates", Misleading DNA Evidence, San Diego: Academic Press, pp. 81–129, ISBN 978-0-12-417214-2, retrieved 2025-11-04{{citation}}: CS1 maint: work parameter with ISBN (link)