PCAF

KAT2B
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1CM0, 1JM4, 1N72, 1WUG, 1WUM, 1ZS5, 2RNW, 2RNX, 3GG3, 4NSQ, 5FE2, 5FE4, 5FDZ, 5FE8, 5FE5, 5FE1, 5FE7, 5FE9, 5FE3, 5FE0, 5FE6
Identifiers
Aliases	KAT2B, CAF, P/CAF, PCAF, lysine acetyltransferase 2B
External IDs	OMIM: 602303; MGI: 1343094; HomoloGene: 20834; GeneCards: KAT2B; OMA:KAT2B - orthologs
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p24.3 Start 20,040,446 bp[1] End 20,154,404 bp[1]
Gene location (Mouse) Chr. Chromosome 17 (mouse)[2] Band 17 C|17 27.86 cM Start 53,873,889 bp[2] End 53,979,748 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in external globus pallidus trabecular bone buccal mucosa cell corpus callosum Achilles tendon subthalamic nucleus pars reticulata amniotic fluid Skeletal muscle tissue of rectus abdominis pars compacta Top expressed in retinal pigment epithelium nasal epithelium olfactory epithelium Epithelium of choroid plexus iris seminal vesicula vestibular membrane of cochlear duct gastrula extensor digitorum longus muscle ankle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription coregulator activity protein binding acyltransferase activity histone acetyltransferase activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein kinase binding chromatin binding lysine N-acetyltransferase activity, acting on acetyl phosphate as donor transcription coactivator activity transcription factor binding histone deacetylase binding acetyltransferase activity protein-containing complex binding peptide-lysine-N-acetyltransferase activity diamine N-acetyltransferase activity Cellular component I band nucleoplasm histone acetyltransferase complex A band actomyosin kinetochore nucleus cytoplasm centrosome microtubule organizing center cytoskeleton protein-containing complex Biological process Notch signaling pathway regulation of protein ADP-ribosylation epigenetic maintenance of chromatin in transcription-competent conformation chromatin remodeling regulation of transcription, DNA-templated negative regulation of cyclin-dependent protein serine/threonine kinase activity rhythmic process N-terminal peptidyl-lysine acetylation transcription, DNA-templated positive regulation of transcription, DNA-templated histone acetylation histone H3 acetylation cellular response to insulin stimulus histone H3-K9 acetylation cell cycle transcription initiation from RNA polymerase II promoter viral process peptidyl-lysine acetylation negative regulation of cell population proliferation positive regulation of transcription by RNA polymerase II internal peptidyl-lysine acetylation regulation of megakaryocyte differentiation protein acetylation positive regulation of transcription of Notch receptor target development of the heart positive regulation of Notch signaling pathway negative regulation of centriole replication limb development protein deubiquitination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8850 18519 Ensembl ENSG00000114166 ENSMUSG00000000708 UniProt Q92831 Q9JHD1 RefSeq (mRNA) NM_003884 NM_001190846 NM_020005 RefSeq (protein) NP_003875 NP_001177775 NP_064389 Location (UCSC) Chr 3: 20.04 – 20.15 Mb Chr 17: 53.87 – 53.98 Mb PubMed search [3] [4]
Wikidata
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P300/CBP-associated factor (PCAF), also known as K(lysine) acetyltransferase 2B (KAT2B), is a human gene and transcriptional coactivator associated with p53.

Several domains of PCAF can act independently or in unison to enable its functions. PCAF has separate acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. PCAF also possesses sites for its own acetylation and ubiquitination.^[5]

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.^[6]

The acetyltransferase activity and cellular location of PCAF are regulated through acetylation of PCAF itself. PCAF may be autoacetylated (acetylated by itself) or by p300. Acetylation leads to migration to the nucleus and enhances its acetyltransferase activity.^[7] PCAF interacts with and is deacetylated by HDAC3, leading to a reduction in PCAF acetyltransferase activity and cytoplasmic localisation.^[8]

PCAF forms complexes with numerous proteins that guide its activity. For example PCAF is recruited by ATF^[9] to acetylate histones and promote transcription of ATF4 target genes.

There are various protein targets of PCAF's acetyltransferase activity including transcription factors such as Fli1,^[10] p53^[11] and numerous histone residues. Hdm2, itself a ubiquitin ligase that targets p53, has also been demonstrated to be a target of the ubiquitin-ligase activity of PCAF.^[5]

PCAF has been shown to interact with:

• Transcription coregulator
• Acetyltransferase

• PCAF protein, human at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
• NURSA C105

This article incorporates text from the United States National Library of Medicine, which is in the public domain.